Lipid Peroxidation Assay Kit

Lipid peroxidation assay protocol summary:

– add TBA reply to samples and necessities, incubate at 95ºC for 60 min, cool in ice bathtub for 10 min
– swap to wells of microplate
– analyze with microplate reader
For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet sooner than analysis.

Lipid Peroxidation (MDA + HNE) Assay Bundle

Lipid peroxidation is a extensively identified occasion of oxidative hurt in cell membranes, lipoproteins, and totally different lipid-containing buildings. Peroxidative modification of unsaturated phospholipids, glycolipids, and ldl ldl cholesterol can occur in a number of reactions. They’re usually triggered by i) free radical species similar to oxyl radicals, peroxyl radicals, and hydroxyl radicals derived from iron-mediated low cost of hydrogen peroxide or ii) non-radical species similar to singlet oxygen, ozone, and peroxynitrite generated by the response of superoxide with nitric oxide.

Malondialdehyde (MDA) and 4-hydroxyalkenals are important toxic byproducts of lipid peroxidation. So, the measurement of the portions of such aldehydes corresponds to an index of lipid peroxidation in vitro and in vivo. 4-Hydroxynonenal (4-HNE) is a severe product of the peroxidative decomposition of ω-6 polyunsaturated fatty acids (PUFA). It possesses cytotoxic, hepatotoxic, mutagenic, and genotoxic properties. Furthermore, elevated ranges of HNE had been current in plasma and diverse organs beneath oxidative stress circumstances. The reality is, MDA is in numerous conditions most likely probably the most ample specific individual aldehyde ensuing from lipid peroxidation. In vitro MDA can alter proteins, DNA, RNA, and loads of totally different biomolecules.

Bioquochem’s LPO assay gear measures MDA and HNE concentrations as an index of lipid peroxidation. Firstly, acid-catalyzed assault on the 3-position of the indole ring initiates the reactions between indoles and aldehydes (MDA and HNE). Due to this, this response offers a diindolylalkane (chromophore) with most absorbance inside the space of 580-620 nm.

In our assay an indol (Reagent A) reacts quickly with MDA and HNE in acidic medium, yielding a chromophore (C) with a extreme molar extinction coefficient at its maximal absorption wavelength of 586 nm.

• Product overview

  Lipid Peroxidation (MDA) Assay Bundle (Colorimetric/Fluorometric) (ab118970) provides a helpful software program for delicate detection of malondialdehyde (MDA).

  Inside the lipid peroxidation assay protocol, the MDA inside the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct might be merely quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA ranges as little as 1 nmol/successfully colorimetrically and 0.1 nmol/successfully fluorometrically.

  The MDA assay can be refered to as a TBARS assay.

  

  Chinese language language protocol on the market. See protocols half beneath.

  For an alternate MDA assay, with out the heating steps required inside the TBARS assay, try MDA assay ab233471.

• Notes

  Lipid peroxidation refers again to the oxidative degradation of lipids. On this course of free radicals take electrons from the lipids (usually in cell membranes), resulting in cell hurt. Quantification of lipid peroxidation is essential to judge oxidative stress. Lipid peroxidation varieties reactive aldehydes similar to malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as pure bi-products. Malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) are typically used as markers of lipid peroxidation, and to assay for oxidative hurt / oxidative stress.

  Related merchandise

  Evaluation the oxidative stress marker and assay info, or the whole metabolism assay info to check additional assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and as well as how one can assay metabolic function in dwell cells using your plate reader.

  Moreover see the favored 4-HNE Assay Bundle ab238538 instead marker of lipid peroxidation and oxidative stress.

  
  How totally different researchers have used Lipid Peroxidation Assay Bundle ab118970

  The MDA/TBARs assay gear has been utilized in publications in a variety of sample types, along with:
  – Human: serum¹, hippocampal principal cell extracts², A375 cultured cell lysates³, plasma and platelet samples⁴
  – Mouse: neuronal cell lysates⁵, coronary coronary heart tissue extract⁶, plasma⁷, cell extracts⁸
  – Rat: hippocampal tissue extracts⁹, cardiomyocyte extracts of cultured cells¹⁰, lung lysates¹¹
  – Pig: serum¹²

  ^{References: 1 – Shen J et al. 2018, 2 – Wang Q et al. 2019, 3 – Luo M et al. 2018,  4 – Mustafa AG et al. 2018, 5 – Murphy Okay et al. 2018, 6 – Guan F et al. 2019, 7 – Costa CRC et al. 2018, 8 – Eleftheriadis T et al. 2019, 9 – Malekiyan et al. 2019, 10 – Zhou Z et al. 2018, 11 – Li L et al. 2018, 12 – Lee SE and Kang KS 2019}

• Platform

  Microplate reader

U-74389G (Lipid peroxidation blocker)
SIH-205-100MG	Stressmarq	100 mg	EUR 208.8
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.

U-74389G (Lipid peroxidation blocker)
SIH-205-500MG	Stressmarq	500 mg	EUR 698.4
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.

Lipid Peroxidation Colorimetric Assay Kit
K454-100	Biovision	each	EUR 652.8

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K739-100	Biovision	each	EUR 698.4

Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit
K2167-100	ApexBio	100 assays	EUR 703.2

Cell Meterâ„¢ Fluorimetric Cellular Lipid Peroxidation Assay Kit
22906-200Tests	AAT Bioquest	200 Tests	EUR 334
Description: Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit
22906	AAT Bioquest	200 Tests	EUR 334

Cell Meterâ„¢ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
15991-200tests	AAT Bioquest	200 tests	EUR 446
Description: Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol.

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit
15991	AAT Bioquest	200 tests	EUR 446

Myeloperoxidase (MPO) Peroxidation Activity Assay Kit
K747-100	Biovision	each	EUR 392.4

OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric)
STA-805	Cell Biolabs	192 assays	EUR 692.4
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample.

Dog Lipid peroxide ELISA kit
E08L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Lipid peroxide ELISA kit
E08L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Lipid peroxide ELISA kit
E08L0263-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lipid peroxide ELISA kit
E02L0263-192T	BlueGene	192 tests	EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Lipid peroxide ELISA kit
E02L0263-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.