Direct PCR

Phire and Phusion Kits

Unit Definition

One unit is outlined as the quantity of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance inside 30 min at 74°C.

Description Code Specification Class Expertise
ALS SARS-CoV-2 RT-PCR Take a look at for surfaces ALS-B01-Adv RUO Meals /Water Virology Detection Actual-Time PCR
ALS SARS-CoV-2 RT-PCR Take a look at for Wastewater ALS-B02-Adv RUO Meals /Water Virology Detection Actual-Time PCR

 

Benefits

  • Fast amplification: The amplification fee of the polymerase is 6kb/min. 1kb fragment will be amplified inside 25min.
  • Lengthy fragment amplification: For plasmid, λ DNA and different straightforward templates, the polymerase can successfully amplify > 20kb. For genome, the polymerase can successfully amplify > 8kb. And for cDNA, the polymerase can successfully amplify > 8kb.
  • Excessive specificity: With scorching begin know-how, the polymerase is 100% inactive beneath 50°C, and might solely be restored by heating at 95°C for 5min.
  • Excessive tolerance to impurities: Samples of entire blood, serum, cultured cells and urine will be straight amplified with out prior DNA purification.

What’s Direct PCR?

Direct PCR (dPCR) is a technique of DNA amplification straight from an animal or plant tissue pattern with out performing DNA isolation and purification steps. This system drastically reduces experimental time, and price in genotyping and high-volume tasks. It additionally supplies a greater possibility when confronted with the challenges of amplifying a really small amount of pattern the place the purification step might doubtlessly result in pattern loss.

In some ways, dPCR works like standard PCR. Each methods depend upon template DNA, primers and a grasp combine or PCR reagents, and each use a thermal cycler for amplification. The first distinction between dPCR and traditional PCR is the tailor-made buffers utilized in dPCR. The modified parts of dPCR allow strong amplification regardless of the presence of PCR inhibitors usually present in crude samples.

 

Direct PCR Reagent
Direct PCR Reagent

ALS SARS-CoV-2 RT-PCR 4G has been developed and produced totally at ALS in Portugal and is designed primarily for export. This new equipment is ready to detect all identified SARS-CoV-2 variants, together with British, Brazilian and South African variants, with out cross-reacting with different identified associated viruses or widespread respiratory pathogens. The selection of the genomic areas used as targets and the sequences of the primers and hydrolysis probes had been primarily based on WHO, CDC and DGS suggestions.

The ALS SARS-CoV-2 RT-PCR 4G equipment is an in vitro diagnostic medical gadget (CE-IVD) that makes use of real-time PCR nucleic acid amplification know-how for the detection of SARS-CoV-2 in medical samples from the higher respiratory tract of people who could or might not be suspected of getting COVID-19.

The equipment makes use of the RT-PCR method, combining amplification of sure areas of the viral genome with its detection by particular hydrolysis probes. The SARS-CoV-2 genome goal areas are extremely conserved and correspond to 2 particular genes: ORF polyprotein (ORF1ab gene) and nucleocapsid phosphoprotein (N gene). The equipment can even detect the gene that codifies the structural protein of the envelope (gene E) particular to the Sarbecovirus subgenus.

 

 

Plant Direct PCR Kit

PD105-02 200 rxn (50 μl/rxn)
EUR 159.5

Plant Direct PCR Kit

PD105-02-200rxns 200 rxns
EUR 165.16

Direct PCR Complete Kit

S700-2 5x100 rcs
EUR 628.8

Direct Blood PCR Kit

E0950-01 100reactions
EUR 69.76
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Blood PCR Kit

E0950-02 500reactions
EUR 277.95
Description: Enables to perform PCR reactions directly from whole blood with no prior DNA extraction or purification

Direct Plant PCR Kit

E0960-01 100reactions
EUR 55.59
Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification

Direct Plant PCR Kit

E0960-02 500reactions
EUR 251.79
Description: Enables to perform PCR reactions directly from plants with no prior DNA extraction or purification

Direct Blood PCR Kit

MBPCR171-100R 1 unit
EUR 267.52
Description: Direct Blood PCR Kit

Direct Blood PCR Kit

MBPCR171-10R 1 unit
EUR 71.73
Description: Direct Blood PCR Kit

Direct Tissue PCR Kit

E0940-01 100reactions
EUR 55.59
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Direct Tissue PCR Kit

E0940-02 500reactions
EUR 251.79
Description: Direct Tissue PCR Kit enables to perform PCR reactions directly from tissue samples with no priorDNA purification

Blood Direct PCR Kit V2

PD103-01 50 rxn
EUR 51.41

Blood Direct PCR Kit V2

PD103-02 200 rxn
EUR 188.5

EzWay Plant Direct PCR Kit

K0568700 1set
EUR 188
Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568700.pdf

Mouse tissue direct PCR kit

EP033 50T
EUR 125

Mouse tissue direct PCR kit

EP033-200T 200T
EUR 125

Mouse tissue direct PCR kit

EP033-50T 50T
EUR 40

Mouse Tissue Direct PCR Kit

RK20736 100RXN
EUR 149.88

amfiSure Direct PCR Lysis Buffer

D0300-010 100ml
EUR 165.6

FastAmp® Plant Direct PCR Kit

4615 1/EA
EUR 396
Description: Intact Genomics FastAmpTM Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps. The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmpTM Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.Highlights:Direct PCR- no need to purify DNASpecially engineered Taq DNA polymerase with highest sensitivity and specificityExtremely short PCR protocol timesMaster mix format with premixed gel loading dye to reduce cross-contamination and sample handlingerrors5x magic enhancer for high GC containing DNA amplificationProduct Includes:FastAmp™ plant direct PCR master mix (2x)Dilution bufferControl primer mix (25 µM each)5x magic enhancerNuclease- free water

EzWay Mouse Tail Direct PCR Kit

K0568600 1set
EUR 188
Description: https://komabiotech.co.kr/labiskoma/datasheet/K0568600.pdf

Mouse Direct PCR Kit (For Genotyping)

B45012 1ml*5 (500 ractions)
EUR 336
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B45013 1ml*20 (2000 ractions)
EUR 1038
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B40013 200 ractions
EUR 250.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Direct PCR Kit (For Genotyping)

B40015 500 ractions
EUR 490.8
Description: The Mouse Direct PCR Kit provides a fast preparation and PCR amplIFication that is specIFically designed for mouse genotyping. Buffer L and Protease Plus rapidly digests mouse tissue to release intact genomic DNA that can be used directly as the template for PCR amplIFication.

Mouse Tissue Direct PCR Kit (With Dye)

10185ES50 50T
EUR 65

 

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