SBI Lentiviral Technologies

SBI Lentiviral Technologies pGF2 mcmv

NEW PRODUCT ALERT!

SBI is labored as much as announce the launch of our subsequent period of pGreenFire signalling pathway reporters! We’ve upgraded these normal lentivectors with a clever design that allows reliable period of regular cell traces and have moreover modified the standard luciferase reporter with crimson firefly luciferase, which opens up the potential of performing a dual-spectral luciferase assay and delivers bigger sensitivity for in vivo features than typical luciferase.

Overview

Make your private pGreenFire 2.zero reporter vector or use as a detrimental administration

With the pGreenFire 2.zero mCMV Cloning & Unfavorable Administration Lentivector & Virus (pGF2-mCMV-rFluc-T2A-GFP-mPGK-Puro), you could benefit from our sturdy pGreenFire 2.zero lentivector know-how to create your private transcriptional response element (TRE) reporter or use pGreenFire 2.zero mCMV as-is as a detrimental administration.

With the pGreenFire 2.zero mCMV Cloning & Unfavorable Administration Lentivector, XhoI and NheI web sites are positioned upstream of a minimal CMV promoter (mCMV) so you could clone in your private TREs. Upon activation, the TREs and mCMV promoter collectively drive co-expression of crimson firefly luciferase and GFP so you could quantitatively measure transcriptional train using every fluorescence and luciferase train.

Alternatively, that you must use the vector as-is as a detrimental administration for any endeavor using pGreenFire 2.zero lentivectors (see info underneath).

What makes our next-gen pGreenFire 2.zero vectors even greater than completely different TRE reporter vectors is the great design, which offers in a constitutive selection cassette for regular cell line period whereas minimizing interference with the upstream TRE. By using a weak/cheap mPGK promoter to drive the antibiotic selection marker (puromycin resistance) and totally arranging the conditional reporter genes, the selection marker is reliably expressed with out compromising conditional expression of rFLuc and GFP.

 

SBI Lentiviral Technologies
SBI Lentiviral Applied sciences

 

pGreenFire Fundamentals

For many who’re not acquainted with our pGreenFire reporters, every 1.zero and a few.zero share a similar core efficiency—transcriptional response components (TREs) are positioned upstream of a minimal CMV promoter (mCMV) and the pGreenFire luciferase-T2A-GFP co-expression cassette. Throughout the absence of transcriptional activation, the mCMV promoter has negligible train  resulting in little- to no- luciferase train or GFP fluorescence (Decide 1). Nonetheless, upon activation of the TREs, comparable to in response to the addition of an inducer, the TREs plus the mCMV promoter drive expression of every luciferase and GFP in a dose-dependent development (Decide 1). The result is the pliability to quantitatively measure pathway activation using luciferase train or whereas imaging using GFP.

As with all of our pGreenFire 2.zero lentivectors, the GreenFire cassette now consists of crimson firefly luciferase (rFLuc), a T2A co-expression element, and GFP. The swap to rFLuc opens up the potential of performing a dual-spectral luciferase assay and likewise delivers bigger sensitivity for in vivo features than typical luciferase.

CD11b Antibody Antibody
ABD2911 100 ug
EUR 438
anti- Antibody^Polyclonal antibody control antibody
LSMab09882 100 ug
EUR 438
Ly1 Antibody Reactive (LYAR) Antibody
20-abx008109
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Anti-Glycolipid Antibody (AGA) Antibody
20-abx004855
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.
Ly1 Antibody Reactive (LYAR) Antibody
20-abx123734
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.
Anti-Glycolipid Antibody (AGA) Antibody
abx036399-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.
Ly1 Antibody Reactive (LYAR) Antibody
20-abx014333
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.
Ly1 Antibody Reactive (LYAR) Antibody
abx033330-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.
Ly1 Antibody Reactive (LYAR) Antibody
abx033330-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.
Anti-Glycoprotein Antibody (GP) Antibody
20-abx319900
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Anti-Glycoprotein Antibody (GP) Antibody
20-abx319901
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Anti-Glycoprotein Antibody (GP) Antibody
20-abx319905
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Anti-Glycoprotein Antibody (GP) Antibody
20-abx319913
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Ly1 Antibody Reactive (LYAR) Antibody
abx234901-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.
Ly1 Antibody Reactive (LYAR) Antibody
20-abx324434
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Ly1 Antibody Reactive (LYAR) Antibody
20-abx311665
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Anti-Glycolipid Antibody (AGA) Antibody
abx230204-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.
Anti-Anti-SEPT6 antibody antibody
STJ11100949 100 µl
EUR 277
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.
Anti-Anti-SEPT9 Antibody antibody
STJ111369 100 µl
EUR 277
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
Anti-Anti-SEPT11 Antibody antibody
STJ111530 100 µl
EUR 277
Anti-Anti-SEPT4 Antibody antibody
STJ112276 100 µl
EUR 277
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.
Anti-Anti-SEPT2 Antibody antibody
STJ25475 100 µl
EUR 277
Anti-Anti-SEPT5 Antibody antibody
STJ25477 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

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