Bio Mag concanvalin a

BioMag Concanavalin A – Canavalia ensiformis

PRODUCT DESCRIPTION

Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are usually found inside the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.

  • Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
  • Detect glycoconjugates in microscopy and circulation cytometry
  • Stain glycoproteins in gels
  • Withstands fixation and permeabilization
  • Number of 10 CF dyes from UV to near-infrared
  • Superior CF dyes are sensible, photostable, and water-soluble

DESCRIPTION Utility

Concanavalin A from Canavalia ensiformis (Jack bean) has been used for creating zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been moreover used inside the preparation of protein loaded microgels to cease enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Packaging

1 g in poly bottle
25, 100, 250 mg in poly bottle

Biochem/physiol Actions

Con A should not be blood group specific nonetheless has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for train. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero bigger aggregates are customary. Con A shows mitogenic train which depends on its diploma of aggregation. Succinylation results in an brisk dimeric sort which stays a dimer above pH 5.6.

Preparation Discover

Extraordinarily purified by specific affinity chromatography.

Analysis Discover

Agglutination train: Determined from serial dilutions of a 1 mg/mL reply (this train is the underside conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.
Concanavalin A (Con A) is probably going one of the crucial usually used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A shows the extreme, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.
BioMag Plus
BioMag Plus

 

Lectins are moreover versatile probes for detecting glycoconjugates in microscopy and circulation cytometric functions and for gel staining of glycoproteins. In neutral and alkaline choices, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic choices (pH beneath 5.0), Con A exists as a dimer. Con A might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles inside the secretory pathway.

Uncover the Correct Stain for Your Utility

Con A and completely different lectins are carbohydrate binding proteins that acknowledge specific sugar moieties on glycoproteins.  The presence and distribution of these targets fluctuate between cell varieties and tissues. In consequence, completely different cell ground stains or completely different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, might produce greater ground staining and is also additional acceptable in your cell kind. Lectin conjugates might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles inside the secretory pathway.

 

 

BioMagPlus Concanavalin A

BP531-10 10 ml
EUR 1399.73
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule

BioMagPlus Concanavalin A

BP531-3 3 ml
EUR 621.95
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule

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Concanavalin A IV

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05-0106-10000 10 g Ask for price

Concanavalin A (ConA)

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Concanavalin A (ConA)

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Description: Available in various conjugation types.

Concanavalin A ELISA Kit

20-abx052181
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  • EUR 4118.40
  • EUR 961.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Concanavalin A (Con A) Antibody

20-abx101152
  • EUR 326.40
  • EUR 159.60
  • EUR 811.20
  • EUR 410.40
  • EUR 292.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Concanavalin A (ConA) Antibody (Biotin)

20-abx271751
  • EUR 460.80
  • EUR 276.00
  • EUR 1262.40
  • EUR 627.60
  • EUR 360.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Concanavalin A Lectin (Con A) - Biotinylated

21510034-1 -
EUR 73.75

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