Bio Mag concanvalin a

BioMag Concanavalin A – Canavalia ensiformis

PRODUCT DESCRIPTION

Concanavalin A (Con A) is a extensively used lectin that selectively binds to the glycoproteins, a-mannopyranosyl and a-glucopyranosy. These are usually found inside the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues.

  • Stain the cell wall of yeast and fungi, and the cell membrane of mammalian cells and tissues
  • Detect glycoconjugates in microscopy and circulation cytometry
  • Stain glycoproteins in gels
  • Withstands fixation and permeabilization
  • Number of 10 CF dyes from UV to near-infrared
  • Superior CF dyes are sensible, photostable, and water-soluble

DESCRIPTION Utility

Concanavalin A from Canavalia ensiformis (Jack bean) has been used for creating zwitterion-immobilized molecularly imprinted polymers (ziMIPs) for protein crystallization. It has been moreover used inside the preparation of protein loaded microgels to cease enzymatic degradation of microgels for monitoring by confocal laser scanning microscope (CLSM).

Packaging

1 g in poly bottle
25, 100, 250 mg in poly bottle

Biochem/physiol Actions

Con A should not be blood group specific nonetheless has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for train. Con A dissociates into dimers at pH 5.6 or beneath. Between pH 5.eight and pH 7.0, Con A exists as a tetramer; above pH 7.Zero bigger aggregates are customary. Con A shows mitogenic train which depends on its diploma of aggregation. Succinylation results in an brisk dimeric sort which stays a dimer above pH 5.6.

Preparation Discover

Extraordinarily purified by specific affinity chromatography.

Analysis Discover

Agglutination train: Determined from serial dilutions of a 1 mg/mL reply (this train is the underside conc. to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C). Requires Type A Human Blood.
Concanavalin A (Con A) is probably going one of the crucial usually used lectins in cell biology. Our Alexa Fluor 488 conjugate of Con A shows the extreme, inexperienced fluorescence of the Alexa Fluor® 488 dye (absorption/emission maxima ~495/519 nm). Alexa Fluor 488 Con A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues.
BioMag Plus
BioMag Plus

 

Lectins are moreover versatile probes for detecting glycoconjugates in microscopy and circulation cytometric functions and for gel staining of glycoproteins. In neutral and alkaline choices, Con A exists as a tetramer with a molecular weight of roughly 104 kDa. In acidic choices (pH beneath 5.0), Con A exists as a dimer. Con A might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles inside the secretory pathway.

Uncover the Correct Stain for Your Utility

Con A and completely different lectins are carbohydrate binding proteins that acknowledge specific sugar moieties on glycoproteins.  The presence and distribution of these targets fluctuate between cell varieties and tissues. In consequence, completely different cell ground stains or completely different lectin conjugates, Wheat Germ Agglutinin (WGA) Conjugates and PNA Lectin Conjugates, might produce greater ground staining and is also additional acceptable in your cell kind. Lectin conjugates might be utilized to selectively stain the cell ground of dwell cells, and stand as much as fixation and permeabilization. When cells are mounted and permeabilized sooner than staining, fluorescent lectins stain every cell ground and organelles inside the secretory pathway.

 

 

Concanavalin A (ConA)
05-0106-1000 1 g
EUR 199
Concanavalin A (ConA)
05-0106-10000 10 g Ask for price
Concanavalin A (ConA)
05-0106-100000 100 g Ask for price
Concanavalin A (ConA)
05-0106-250 250 mg
EUR 88
Concanavalin A IV
abx082153-25mg 25 mg
EUR 258
  • Shipped within 5-10 working days.
BioMagPlus Concanavalin A
BP531-10 10 ml
EUR 1166.44
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule
BioMagPlus Concanavalin A
BP531-3 3 ml
EUR 518.29
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule
Concanavalin A (Con A) Antibody
20-abx101152
  • EUR 272.00
  • EUR 133.00
  • EUR 676.00
  • EUR 342.00
  • EUR 244.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Concanavalin A, CF350 conjugate
29015 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF488A conjugate
29016 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF594 conjugate
29017 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF633 conjugate
29018 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF640R conjugate
29019 5x1mg
EUR 209
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF680 conjugate
29020 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF770 conjugate
29058 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF405M conjugate
29074 5x1mg
EUR 219
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF405S conjugate
29075 5x1mg
EUR 219
Description: Minimum order quantity: 1 unit of 5x1mg
Concanavalin A, CF750 conjugate
29080 5x1mg
EUR 240
Description: Minimum order quantity: 1 unit of 5x1mg

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