Proteinase Okay (Lyophilized)

Protected at Room Temperature and Simple to Use

• Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
• Retains >80% prepare at temperatures as rather a lot as 60°C
• No resuspension or thawing ahead of use
• Cat.# MC5005, MC5008 equipped at a highlight of 20mg/ml

 

Proteinase Okay – considered one of many sometimes used enzymes in molecular biology.

 

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

 

Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Keep in mind

• The truly useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in route of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Protected over an enormous pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the best top of the range product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Decisions

• Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
• Excessive prepare and distinctive purity.
• Lively at excessive temperatures (as rather a lot as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in a wide range of options.
• Protected over an enormous pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
• Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
• Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

• Extraction of DNA and RNA from utterly completely completely different beginning gives.
• Purification of goal provides from contaminating proteins.
• Elimination of DNases and RNases all by nucleic acids isolation.

 

Proteinase K
BIO-37037	Bioline	100mg	Ask for price

Proteinase K
BIO-37039	Bioline	1000mg	Ask for price

Proteinase K
Z5050002	Biochain	100 mg	EUR 172

Proteinase K
MB086-100MG	EWC Diagnostics	1 unit	EUR 71.16
Description: Proteinase K

Proteinase K
MB086-10MG	EWC Diagnostics	1 unit	EUR 7.75
Description: Proteinase K

Proteinase K
MB086-1G	EWC Diagnostics	1 unit	EUR 554.5
Description: Proteinase K

Proteinase K
RM2957-100MG	EWC Diagnostics	1 unit	EUR 93.9
Description: Proteinase K

Proteinase K
RM2957-10MG	EWC Diagnostics	1 unit	EUR 10.43
Description: Proteinase K

Proteinase K
RM2957-1G	EWC Diagnostics	1 unit	EUR 844.84
Description: Proteinase K

Proteinase K
EQ023-1mL	ELK Biotech	1mL	EUR 16

Proteinase K
E4350-01	EURx	1ml	EUR 23.98
Description: Solution 20 mg/ml

Proteinase K
E4350-02	EURx	5x1ml	EUR 107.91
Description: Solution 20 mg/ml

Proteinase K
GE0990	Glentham Life Sciences	25mg	EUR 347.58

Proteinase K
G029	ABM	1.0 ml	EUR 85
Description: Proteinase K is a serine protease often used to digest protein and to remove contamination in nucleic acid preparations. It does so by inactivating nucleases which could otherwise cause the break down of DNA and/or RNA.

Proteinase K
EQ023	ELK Biotech	1mL	EUR 16

PROTEINASE K
IB05402	IBI Scientific	200MG	EUR 158.37
Description: (FR)

Proteinase K
GE0990-1	Glentham Life Sciences	1	EUR 379.6

Proteinase K
GE0990-100	Glentham Life Sciences	100	EUR 63.3

Proteinase K
GE0990-100MG	Glentham Life Sciences	100 mg	EUR 112.8