Cusao Proteinase k

Proteinase K, recombinant

Proteinase Okay (Lyophilized)

For Elementary Digestion of Protein in Pure Samples

  • Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
  • Purified to take away RNase and DNase actions

Protected at Room Temperature and Simple to Use

  • Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% prepare at temperatures as rather a lot as 60°C
  • No resuspension or thawing ahead of use
  • Cat.# MC5005, MC5008 equipped at a highlight of 20mg/ml

 

Proteinase Okay – considered one of many sometimes used enzymes in molecular biology.

Superb completely different for protein digestion in assorted options.

  • BLIRT – expert in Proteinase Okay manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing all through the best top of the range product with a really low batch-to-batch variability.
  • Big elements, the prospect of white labeling, and portioning service – liquid selection all through the quantity on the shopper’s request (as rather a lot as a wide range of million samples month-to-month).

 

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

 

Proteinase K, recombinant
Proteinase Okay, recombinant



Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Keep in mind

• The truly useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in route of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay
• Protected over an enormous pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

 

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the best top of the range product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Decisions

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Excessive prepare and distinctive purity.
  • Lively at excessive temperatures (as rather a lot as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in a wide range of options.
  • Protected over an enormous pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from utterly completely completely different beginning gives.
  • Purification of goal provides from contaminating proteins.
  • Elimination of DNases and RNases all by nucleic acids isolation.

 

Proteinase K
G029 1.0 ml
EUR 142
Proteinase K
GE0990-100MG 100 mg
EUR 94
Proteinase K
GE0990-1G 1 g
EUR 413
Proteinase K
GE0990-250MG 250 mg
EUR 154
Proteinase K
GE0990-25MG 25 mg
EUR 58
Proteinase K
GE0990-500MG 500 mg
EUR 249
Proteinase K
abx082041-100mg 100 mg
EUR 258
  • Shipped within 5-10 working days.
Proteinase K
abx098140-1ml 1 ml
EUR 189
  • Shipped within 5-10 working days.
Proteinase K
K1037-1 1 ml
EUR 67
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Proteinase K
K1037-5 5 ml
EUR 108
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Proteinase K
P2180-010 100mg
EUR 182
Proteinase K
P2180-050 500mg
EUR 374
Proteinase K
P2180-100 1g
EUR 523
Proteinase K
Z5050002 100 mg
EUR 171
Description: No genetic downstream application can do without the good old Proteinase K.
Proteinase K Solution
BIO-37084 5ml @ 20mg/ml Ask for price
Proteinase K Solution
BIO-37085 5 x 5ml @ 20mg/ml Ask for price
Proteinase K solution
GZ5904-100ML 100 ml
EUR 907
Proteinase K solution
GZ5904-25ML 25 ml
EUR 349
PROTEINASE K SOLUTION
501-PK 5 ml
EUR 152
Description: Genomic PCR Grade Proteinase K Solution

Leave a Comment

Your email address will not be published. Required fields are marked *