Dedication of the relative molecular mass distribution of the ultrafiltration fractionation product of lignosulfonate by superior polymer chromatography.
The results of testing situations, reminiscent of pH and ionic energy of the cell part, on the relative molecular mass obtained by superior polymer chromatography (APC) have been investigated systematically.
- The non-size exclusion results have been mentioned for lignosulfonate and its ultrafiltration fractionation product. The relative molecular mass distribution of lignosulfo-nate was well-characterized by an APC system utilizing three columns in collection, with a 10 μ L injection quantity and 0.1 mol/L NaNO3 because the aqueous cell part, at a circulation price of 0.5 mL/min.
- The relative molecular mass distribution is within the eight molecular mass ranges. Optimized check situations have been used to characterize the ultrafiltration fractionation product of lignosulfonate. The obtained quantity common relative molecular lots (Mn) of the three elements have been 40410, 12208, and 1516 Da, indicating that lignosulfonate was effectively separated into three fractions by ultrafiltration membrane separation.
- The APC method offered herein gives the premise for additional software of the fractional separation merchandise of lignosulfonate.
) MicroElute GEL/PCR Purification Kit (300prep) |
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| FAEPK-001-2 | Favorgen | 300 preps | EUR 229 |
) GEL/PCR Purification Mini Kit (100prep) |
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| FAGCK-001 | Favorgen | 100 preps | EUR 129 |
) GEL/PCR Purification Mini Kit (300prep) |
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| FAGCK-001-1 | Favorgen | 300 preps | EUR 172 |
 Gel and PCR DNA Purification kit |
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| K1455-250 | Biovision | 250 Preps | EUR 480 |
Gel and PCR DNA Purification kit |
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| K1455-50 | Biovision | 50 Preps | EUR 206 |
 96-Well Plate PCR Products Purification Kit |
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| BS365 | Bio Basic | 5XPlates, 480prep | EUR 250.31 |
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96-Well Plate PCR Products Purification Kit |
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| BS3652 | Bio Basic | 2xPlates, 192prep | EUR 155.16 |
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 EZ-10 Spin Column PCR Products Purification Kit |
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| BS363 | Bio Basic | 50Preps | EUR 76.64 |
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EZ-10 Spin Column PCR Products Purification Kit |
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| BS364 | Bio Basic | 100preps | EUR 95.68 |
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EZ-10 Spin Column PCR Products Purification Kit |
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| BS664 | Bio Basic | 250preps | EUR 166.58 |
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 ISOLATE II PCR & Gel Kit |
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| BIO-52058 | Bioline | 10 preps | Ask for price |
ISOLATE II PCR & Gel Kit |
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| BIO-52059 | Bioline | 50 preps | Ask for price |
ISOLATE II PCR & Gel Kit |
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| BIO-52060 | Bioline | 250 preps | Ask for price |
) T-Pro Gel/PCR DNA Purification Maxi Kit (10) |
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| RB94-NEL010 | T-Pro Biotechnology | 10preps/Kit | EUR 161 |
) T-Pro Gel/PCR DNA Purification Mini Kit (100) |
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| RB94-NES100 | T-Pro Biotechnology | 100preps/Kit | EUR 161 |
) T-Pro Gel/PCR DNA Purification Mini Kit (250) |
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| RB94-NES250 | T-Pro Biotechnology | 250preps/Kit | EUR 222 |
 Agarose, Low melting gel |
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| AB0015 | Bio Basic | 10g | EUR 80.45 |
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) GEL Purification Mini Kit (50prep) |
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| FAGPK-001 | Favorgen | 50 preps | EUR 120 |
) GEL Purification Mini Kit (200prep) |
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| FAGPK-001-1 | Favorgen | 200 preps | EUR 169 |
) GEL Purification Mini Kit (300prep) |
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| FAGPK-001-2 | Favorgen | 300 preps | EUR 189 |
) MicroElute GEL Purification Kit (50prep) |
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| FAMGK-001 | Favorgen | 50 preps | EUR 126 |
) MicroElute GEL Purification Kit (200prep) |
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| FAMGK-001-1 | Favorgen | 200 preps | EUR 187 |
 PCR Purification Kit |
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| 20-abx098084 | Abbexa |
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 Hi-Bind? T-Gel-Agarose |
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| M1313-1 | Biovision | EUR 131 | |
Hi-Bind? T-Gel-Agarose |
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| M1313-10 | Biovision | EUR 218 | |
Hi-Bind? T-Gel-Agarose |
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| M1313-100 | Biovision | EUR 1501 | |
 Ni-NTA His-Tag Purification Agarose |
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| HY-K0210 | MedChemExpress | 50 mL | EUR 652 |
 Zenoquick PCR Purification Kit |
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| Z3002-050 | GenDepot | 50 preps | EUR 219 |
Zenoquick PCR Purification Kit |
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| Z3002-200 | GenDepot | 4x50 preps | EUR 631 |
 RFLP FAdV II PCR kit |
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| PCR-V037-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of RFLP FAdV II |
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RFLP FAdV II PCR kit |
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| PCR-V037-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of RFLP FAdV II |
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) 96-well GEL Purification Kit (1 prep) |
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| FAGKE-96001 | Favorgen | 1 prep | EUR 158 |
) 96-well GEL Purification Kit (2 prep) |
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| FAGKE-96002 | Favorgen | 2 preps | EUR 213 |
) 96-well GEL Purification Kit (4 prep) |
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| FAGKE-96004 | Favorgen | 4 preps | EUR 317 |
) AmbiClean Kit (PCR & Gel) |
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| GV05 | GeneOn | 5 preps | Ask for price |
AmbiClean Kit (PCR & Gel) |
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| GV100 | GeneOn | 100 preps | EUR 128 |
AmbiClean Kit (PCR & Gel) |
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| GV200 | GeneOn | 200 preps | EUR 189 |
AmbiClean Kit (PCR & Gel) |
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| GV50 | GeneOn | 50 preps | EUR 91 |
 Human Kinase Library II |
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| HKIN-II | Real Time Primers | 1 set | EUR 450 |
 Human Drug Detoxication II |
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| HDTX-II | Real Time Primers | 1 set | EUR 645 |
 PCR DNA Extraction and Purification |
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| EP10013 | Neuromics | 100 Tests | EUR 297 |
) PCR Purification Kit (100 preps) |
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| 9K-006-0003 | Bio Basic | 100preps | EUR 224 |
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) PCR Purification Kit (200 preps) |
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| 9K-006-0004 | Bio Basic | 200preps, 200prep | EUR 311 |
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 Bacterial DNA Purification Kit II |
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| K1457-250 | Biovision | 250 Preps | EUR 581 |
Bacterial DNA Purification Kit II |
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| K1457-50 | Biovision | 50 Preps | EUR 318 |
 Human Cytokine Primer Library II |
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| HCA-II | Real Time Primers | 1 set | EUR 450 |
 Mouse Cytokine Primer Library II |
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| MCA-II | Real Time Primers | 1 set | EUR 450 |
 Rat Cytokine Primer Library II |
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| RCA-II | Real Time Primers | 1 set | EUR 548 |
 Human Colon Cancer Primer Library II |
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| HCCP-II | Real Time Primers | 1 set | EUR 548 |
 Human DNA Repair Primer Library II |
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| HDRL-II | Real Time Primers | 1 set | EUR 548 |
 Mouse DNA Repair Primer Library II |
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| MDRL-II | Real Time Primers | 1 set | EUR 645 |
 Human Interferon Type II Signaling Primer Library |
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| HIFN-II | Real Time Primers | 1 set | EUR 548 |
 Human Cancer Driver Gene II Primer Library |
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| HCDG-II | Real Time Primers | 1 set | EUR 645 |
 PCR DNA extraction and purification kit |
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| K1444-100 | Biovision | EUR 338 | |
 SeqPure PCR Purification Kit, 5 ml |
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| Z1510001 | Biochain | 5 ml | EUR 304 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 SeqPure PCR Purification Kit, 50 ml |
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| Z1510002 | Biochain | 50 ml | EUR 899 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 SeqPure PCR Purification Kit, 500 ml |
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| Z1510003 | Biochain | 500 ml | EUR 5936 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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 AffiPure Ni-NTA Agarose Bead, His-Tagged protein purification |
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| N3505-005 | GenDepot | 5ml | EUR 169 |
AffiPure Ni-NTA Agarose Bead, His-Tagged protein purification |
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| N3505-025 | GenDepot | 25ml | EUR 448 |
AffiPure Ni-NTA Agarose Bead, His-Tagged protein purification |
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| N3505-100 | GenDepot | 100ml | EUR 1562 |
AffiPure Ni-NTA Agarose Bead, His-Tagged protein purification |
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| N3505-500 | GenDepot | 500ml | EUR 6125 |
 Recombinant Protein A+G-Agarose suspension for Ig's purification |
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| PRAG15-AS-1 | Alpha Diagnostics | 1 ml | EUR 202 |
Recombinant Protein A+G-Agarose suspension for Ig's purification |
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| PRAG15-AS-5 | Alpha Diagnostics | 5 ml | EUR 651 |
 HyAgarose? HR Agarose, PCR Grade, 100g |
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| R9012HR-100g | ACTGene | EUR 332 | |
 HyAgarose? HR Agarose, PCR Grade, 10g |
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| R9012HR-10g | ACTGene | EUR 83 | |
 HyAgarose? HR Agarose, PCR Grade, 500g |
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| R9012HR-500g | ACTGene | EUR 1141 | |
 HyAgarose? HR Agarose, PCR Grade, 50g |
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| R9012HR-50g | ACTGene | EUR 202 | |
 AffiPure Ni-NTA Agarose Bead,For His-Tagged Protein Purification |
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| N3505-010 | GenDepot | 10ml | EUR 317 |
AffiPure Ni-NTA Agarose Bead,For His-Tagged Protein Purification |
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| N3505-050 | GenDepot | 50ml | EUR 833 |
) Cys-link agarose gel coupling buffer kit (5-10 peptides) |
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| 110400-CB | Alpha Diagnostics | 1 kit | EUR 225 |
) Gel and PCR Extraction System (100 preps) |
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| 9K-006-0007 | Bio Basic | 100preps | EUR 150.05 |
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) Gel and PCR Extraction System (200 Preps) |
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| 9K-006-0008 | Bio Basic | 200preps, 200prep | EUR 219.65 |
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, ELISA, and affinity column) Custom Affinity Purification of antibodies (Coupling of antigen to Agarose, Ab purification (10 ml), ELISA, and affinity column |
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| AFFI-I | Alpha Diagnostics | 1 | EUR 773 |
 E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies |
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| EC11-G | Alpha Diagnostics | 1 ml | EUR 286 |
) Preactivated Cys-Link-Affinity Gel Agarose (for Cys-containing peptides/proteins) |
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| 110100-CG | Alpha Diagnostics | 5 ml | EUR 286 |
) Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins) |
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| 110200-NG | Alpha Diagnostics | 5 ml | EUR 286 |
-Agarose affinity gel for removing KLH antibodies) Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies |
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| KLH11-G | Alpha Diagnostics | 1 ml | EUR 225 |
 MULTIPLEX KIT PCR MASTITIS PCR kit |
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| PCR-MPX218-48D | Bioingentech | 50T | EUR 425.8 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS |
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MULTIPLEX KIT PCR MASTITIS PCR kit |
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| PCR-MPX218-96D | Bioingentech | 100T | EUR 521.5 |
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Description: An conventional PCR kit for detection of MULTIPLEX KIT PCR MASTITIS |
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) PCR Purification Solutions Kit (for 50x 96 well-plates) |
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| 9K-006-0005 | Bio Basic | 50, 100ug | EUR 451.5 |
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 Agarose |
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| G060-1 | ABM | 100 g | EUR 176 |
Agarose |
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| G060-2 | ABM | 500 g | EUR 369 |
Agarose |
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| abx082006-100g | Abbexa | 100 g | EUR 244 |
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Agarose |
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| abx082007-100g | Abbexa | 100 g | EUR 258 |
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Agarose |
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| abx082008-100g | Abbexa | 100 g | EUR 230 |
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Agarose |
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| 20-abx098147 | Abbexa |
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Agarose |
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| 20-abx186369 | Abbexa |
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Agarose |
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| Z5050007 | Biochain | 500 g | EUR 615 |
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Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time. |
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Agarose |
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| Z5050013 | Biochain | 100 g | EUR 189 |
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Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time. |
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Prevalence and molecular characterization of Sarcocystis infections of retail beef merchandise from central China.
Cattle are the intermediate hosts for 5 Sarcocystis species together with S. hominis and S. heydorni, which additionally infect people. To analyze the prevalence of Sarcocystis infections in beef merchandise from 17 cities within the Henan Province of central China, 62 uncooked beef samples from markets have been collected and analyzed for Sarcocystis presence by way of muscle squashing microscopic statement, histological part examination, and molecular characterization with 18S rRNA gene sequencing.
Sarcocystis have been detected in a complete of 20 of the meat samples. 4 species have been recognized that comprised S. cruzi, S. rommeli, S. heydorni, and S. hirsuta, with S. cruzi because the dominant species. As well as, seven of the 20 contaminated samples have been contaminated with two or three species. Evaluation of the 18S rRNA sequences recovered from these samples recommended little or no genetic variety inside every species.
This research represents the primary molecular identification of Sarcocystis species an infection in retail beef merchandise from China. These findings will present beneficial data for evaluating the potential public well being threat of bovine Sarcocystis species infections and the management of sarcocystosis in cattle
Versatile docking-based molecular dynamics simulation of pure product compounds and Ebola virus Nucleocapsid (EBOV NP): a computational method to find new drug for combating Ebola.
Ebola nonetheless stays as one of the problematic infectious illnesses in Africa with a excessive price of mortality. Though this illness has been identified for an virtually half-century, there aren’t any vaccines and medicines obtainable available in the market to deal with Ebola. Zaire ebolavirus (EBOV), a single-stranded RNA virus which belongs to Filoviridae household and Mononegavirales order, is one of many virus inflicting Ebola. As considered one of seven proteins that EBOV encodes, Ebola virus nucleoprotein (EBOV NP) performs an crucial function in EBOV proliferation cycle. Subsequently, the event of a brand new Ebola remedy could be focused in the direction of EBOV NP.
RESULTS:
On this work, we screened about 190,084 pure product compounds from ZINC15 database by means of in silico digital screening and versatile docking simulation. Moreover, the bioavailability and toxicity prediction have been performed as effectively. Two finest ligands in response to the simulation and prediction assessments have been progressed into the molecular dynamics simulation.
CONCLUSIONS:
In the long run, we discovered that our proposed ligands, particularly α-lipomycin (ZINC56874155) and 3-(((S)-1-amino-1,2,3,4-tetrahydroisoquinolin-5-yl)methyl)-5-((5-((5R,7S)-5,7-dihydroxy-3-oxodecyl)-2-hydroxyphenoxy) methyl)pyrrolo[3,4-b]pyrrol-5-ium (ZINC85628951), confirmed the promising outcomes to be developed as a lead compounds for treating Ebola. Subsequently, an experimental research is required to validate their inhibition actions towards EBOV NP
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