mygentaur.com

Molecular dynamics simulations reveal essential residues

Steered molecular dynamics simulations reveal important residues for (un)binding of substrates, inhibitors and a product to the malarial M1 aminopeptidase.

Malaria is a life-threatening sickness unfold by mosquitoes. Plasmodium falciparum M1 alanyl aminopeptidase (PfM1-AAP) is a promising aim for the remedy of malaria. The simply recently solved crystal buildings of PfM1-AAP revealed that the buried full of life web page could also be accessed through two channel openings: a short N-terminal channel with th

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mygentaur.com

e measurement of Eight Å and a protracted C-terminal channel with the scale of 30 Å. It is unclear, nonetheless, how substrates and inhibitors migrate to the full of life web page and a product of cleavage leaves.

Proper right here, we analysis the molecular mechanism of substrate and inhibitor migration to the full of life web page and the product launch using steered molecular dynamics simulations. We acknowledged a stepwise passage of substrates and inhibitors throughout the C-terminal channel of PfM1-AAP, involving (I) ligand recognition on the opening of the channel, (II) ionic translation to the ‘water reservoir’, (III) ligand reorientation throughout the ‘water reservoir’ and (IV) passage in an acceptable conformation into the full of life web page. Endorsed by enzymatic analysis of purposeful recombinant PfM1-AAP and mutagenesis analysis, our novel ligand-residue binding group analysis has acknowledged the purposeful residues controlling ligand migration all through the C-terminal channel of PfM1-AAP.
Furthermore, from unbinding simulations of the Arg product we advise a value repulsion as a result of the driving energy to expel the product out from the N-terminal channel of PfM1-AAP. Our work paves the best way by which within the path of the design of a novel class of PfM1-AAP inhibitors based mostly totally on stopping substrate entry to the full of life web page.

Control for ER, 4 cases (1.5mm)

ERC081 1
EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

Control for HER2, 4 cases (1.5mm)

HRC081 1
EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

Control for PR, 3 cases (1.5mm)

PRC061 1
EUR 85
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

Control for Ckit, 6 samples (1.5mm)

CKIT061 1
EUR 85
Description: Gastrointestinal stroma tumors, 6 cores, 3 cases in duplicates showing strong, moderate and no expression of cKit molecule.

Control for DOG1, 3 cases (1.5mm)

DOG1061 1
EUR 85
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-100 100 Slides
EUR 706

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-25 25 Slides
EUR 232

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-5 5 Slides
EUR 98

Thiostatin Rat protein, control for western

TSTN11-C 100 ul
EUR 286

Control/Blocking peptide for Human WNT5a

WNT511-P 100 ug
EUR 164

CMV-Null control Adenovirus, in vivo ready

AVP-Null-PBS 1x1011 IFU/ml x 50ul
EUR 552
Description: Pre-made Null-control Adenovirus, provided in PBS solution.

Ferret IgG (Control, non-immune, isotype control), semi-pure for ELISA

20021-1 100 ug
EUR 225

Elk IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20024-1 100 ug
EUR 225

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20024-2 100 ug
EUR 225

Bison IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20025-1 100 ug
EUR 225

Raccoon IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20026-1 100 ug
EUR 225

Skunk IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20027-1 100 ug
EUR 225

Recombinant (E. Coli) Mouse Adenovirus (MADV) Hexon Protein (hxn) Control for Western Blot

MADV11-C 100 ul
EUR 286

Adenovirus Rapid Test Control Pack (2 X 1.5mL)

KZMC035 2 X 1.5mL
EUR 97.84
Description: Please contact Gentaur in order to receive the datasheet of the product.

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column

FAPDE-holder-for-ion-exchange 1 prep
EUR 158

SLLK, Control Peptide for TSP1 Inhibitor(TFA)

HY-P0301A 5mg
EUR 380

Positive Control for Anti-Human CYP1A2 Antibody

P1A2CON 100 uL
EUR 125

Positive Control for Anti-Rat CYP2B1 Antibody

P2B1PTCON 100 uL
EUR 125

Positive Control for Anti-Rat CYP2C Antibody

P2CCON 100 uL
EUR 125

Positive Control for Anti-Rat CYP2D Antibody

P2DCON 100 uL
EUR 125

Positive Control for Anti-Human CYP2E1 Antibody

P2E1PTCON 100 uL
EUR 125

Positive Control for Anti-Rat CYP3A2 Antibody

P3A2PTCON 100 uL
EUR 125

Positive Control for Anti-Human CYP3A Antibody

P3ACON 100 uL
EUR 125

Positive Control for Anti-Human CYP1B1 Antibody

PH1B1CON 100 uL
EUR 125

Positive Control for Anti-Rat MEH Antibody

MEHCON 100 uL
EUR 125

Mouse IgG (-ve control for flow cytometry)

20008-1-100 100 tests
EUR 103

Rabbit IgG (-ve control for flow cytometry)

20009-1-100 100 tests
EUR 103

Rec. Human CD21 protein control for WB

CD21-C 100 ul
EUR 286

Mouse Ceruloplasmin protein control for western blot

CP16-C 100 ul
EUR 286

Rat Ceruloplasmin protein control for western blot

CP17-C 100 ul
EUR 286

Control Sections for FD NeuroSilver™ Kit

PCS101 each
EUR 58

Purified, Bovine S100 protein control for WB

S1001-C 100 ul
EUR 286

Purified, Human S100 protein control for WB

S1002-C 100 ul
EUR 286

Chicken Egg Ovalbumin protein control for Western

OVA11-C 100 ul
EUR 286

Control/Blocking peptide for Mouse Vimentin (Vim)

VIM11-C 100 ug
EUR 164

Control/Blocking peptide for Human WNT-1

WNT111-P 100 ug
EUR 457

Purified Flagellin (salmonella) protein control for WB

FLGN17-C 100 ul
EUR 286

Mouse negative control sera (purified) for IHC

NCM802M 7 ml
EUR 199

Control/Blocking peptide for Ephrin-B2 antibody

NIVE2B-C 100 ug
EUR 164

Purified bovine Lactoferrin protein control for Western

LTF13-C 100 ul
EUR 286

Haptoglobin Human Plasma protein control for WB

HGLB18-C 100 ul
EUR 286

Human Hemopexin purified protein control for Western

HPEX11-C 100 ul
EUR 286

Hemopexin Mouse, purified protein control for Western

HPEX16-C 100 ul
EUR 286

Bovine Adenosine deaminase protein control for Western

ADA11-C 100 ul
EUR 286

Human Apolipoprotein B protein control for WB

APOB21-C 100 ul
EUR 286

Control siRNA Vector (pGB-control)

9500C-20
EUR 338

Positive Control for Anti-Rat CYP1A1/1A2 Antibody

P1ACON 100 uL
EUR 125

Positive Control for Anti-Rat CHP2B1/2 Antibody

P2B12PTCON 100 uL
EUR 125

Positive Control for Anti-Human Cytochrome b5 Antibody

Cb5CON 100 uL
EUR 125

Mouse Anti-Cardiolipin Ig's -ve control for ELISA

5502 1 ml
EUR 225

Recombinant Beta Lactamase protein control for Western blot

BLAC11-C 100 ul
EUR 286

Purified human Catalase (CATL) protein control for WB

CATL11-C 100 ul
EUR 286

Chicken Egg Conalbumin (ovotransferrin) protein control for Western

CECA15-C 100 ul
EUR 286

Chicken Egg white proteins total control for Western

CEGW12-C 100 ul
EUR 286

Chicken Egg yellow proteins total control for Western

CEYP12-C 100 ul
EUR 286

Chicken Ovomucoid (trypsin inhibitor) protein control for Western

COVM13-C 100 ul
EUR 286

Purified Cholera Toxin protein control for Western Blot

CTOX11-C 100 ul
EUR 286

Control sections for FD NeuroApop™ Kit (frozen)

PCS102- each
EUR 73

Control sections for FD NeuroApop™ Kit (paraffin)

PCS102-1 each
EUR 67.03
Description: Best quality products

Control sections for FD NeuroApop™ Kit (frozen)

PCS102-2 each
EUR 67.12
Description: Best quality products

Human Pancreatic Amylase protein control for Western blot

PAMY13-C 100 ul
EUR 286

Human Prothrombin (Factor II) protein control for Western

PRTN11-C 100 ul
EUR 286

Mouse Prothrombin (Factor II) protein control for Western

PRTN13-C 100 ul
EUR 286

Human Salivary Amylase protein positive control for WB

SAMY16-C 100 ul
EUR 286

Purified Human Survivin protein for WB +ve control

SURV11-C 100 ul
EUR 286

Purified, recombinant Human TACE control protein for WB

TACE11-C 100 ul
EUR 286

Purified Human Neutrophil Elastase protein control for WB

ESTE11-C 100 ul
EUR 286

Myoglobin, Human Cardiac, purified protein control for western

MYG17-C 100 ul
EUR 286

Purified Nipah virus Glycoprotein control for Western Blotting

NIV11-C 100 ul
EUR 286

Purified Nipah virus Nucleoprotein control for Western Blotting

NIV21-C 100 ul
EUR 225

Purified Lassa fever Nucleoprotein control for Western Blotting

LFNP15-C-10 100 ul
EUR 286

Control/Blocking peptide for Mouse Histone deacetylase 1

HDAC111-P 100 ug
EUR 164

Rabbit Beta Actin (muscle) protein control for Western

ACTB15-C 100 ul
EUR 324

Chicken Serum Albumin protein control for Western blot

ALBC15-C 100 ul
EUR 286

Monkey (Rhesus) Serum Albumin protein control for WB

ALBMK21-C 100 ul
EUR 286

The CXCL12 (SDF-1)/CXCR4 chemokine axis: Oncogenic properties, molecular concentrating on, and synthetic and pure product CXCR4 inhibitors for many cancers treatment.

Chemokine 12 (CXCL12), usually generally known as stromal cell derived factor-1 (SDF-1) and a member of the CXC chemokine subfamily, is ubiquitously expressed in a number of tissues and cell varieties.
It interacts notably with the ligand for the transmembrane G protein-coupled receptors CXCR4 and CXCR7. The CXCL12/CXCR4 axis takes half in a sequence of physiological, biochemical, and pathological course of, akin to irritation and leukocyte trafficking, cancer-induced bone ache, and postsurgical ache, and likewise is a key problem throughout the cross-talking between tumor cells and their microenvironment.
Aberrant overexpression of CXCR4 is important for tumor survival, proliferation, angiogenesis, homing and metastasis. On this overview, we summarized the operate of CXCL12/CXCR4 in most cancers, CXCR4 inhibitors beneath scientific analysis, and pure product CXCR4 antagonists. In conclusion, the CXCL12/CXCR4 signaling is critical for tumor progress and concentrating on the pathway might symbolize an environment friendly technique to creating novel treatment in most cancers remedy

Affect on the mechanical properties of kind I collagen of intra-molecular lysine-arginine derived superior glycation end-product cross-linking.

Non-enzymatic superior glycation end product (AGE) cross-linking of collagen molecules has been hypothesised to result in necessary modifications to the mechanical properties of the connective tissues all through the physique, doubtlessly resulting in various age related sicknesses.
We now have investigated the impression of two of these cross-links, glucosepane and DOGDIC, on the tensile and lateral moduli of the collagen molecule through utilizing a steered molecular dynamics technique, using beforehand acknowledged preferential formation web sites for intra-molecular cross-links.
Our outcomes current that the presence of intra-molecular AGE cross-links will improve the tensile and lateral Youthful’s moduli throughout the low strain space by between 3.0-8.5% and a few.9-60.3% respectively, with little impression exhibited at better strains

Utility of FT/IR-ATR vibrational spectroscopy to reveal protein molecular building of feedstock and co-merchandise from Canadian and Chinese language language canola processing in relation to microorganism bio-degradation and enzyme bio-digestion.

The principal aim of this analysis was to make use of FT/IR-ATR vibrational spectroscopy to look at the connection between rumen dry matter (DM) and protein degradation, rumen undegraded protein (RUP) intestinal digestion and processing induced protein molecular building modifications in feedstock (canola oil seeds) and co-products (canola meal) from bio-oil processing from fully totally different crushing crops in Canada and China.
  • The rumen DM and protein degradation, rumen undegraded protein intestinal digestion and protein molecular building affected by bio-oil processing have been examined using in situ, three step in vitro digestion and Fourier rework infrared (FT/IR) molecular spectroscopy methods, respectively. The outcomes confirmed that the protein molecular building; α-helix peak and α-helix to β-sheet peak ratio had an in depth affiliation with rumen DM and protein degradation and rumen undegraded protein intestinal digestibility.
  • Quite a few regression analyses confirmed that protein β-sheet peak and α-helix to β-sheet peak ratio spectral depth could be utilized to predict rumen DM and protein degradation, whereas intestinal digestibility of rumen undegraded protein could also be predicted by α-helix peak and β-sheet peak.
  • In conclusion, the co-product canola meal from bio-oil processing is an environment friendly provide of intestinally digestible protein. Rumen DM and protein degradation and intestinal digestibility of rumen undegraded protein are related to the protein molecular buildings of the co-products affected by modifications all through bio-oil processing

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1 1MG
EUR 168
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011 500uG
EUR 367
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012 500uG
EUR 383
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013 10ST
EUR 417
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014 10ST
EUR 466
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015 500uG
EUR 466
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016 10ST
EUR 523
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017 500uG
EUR 433
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020 1MG
EUR 314
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1 20ST
EUR 418
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021 1MG
EUR 344
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1 20ST
EUR 448
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022 1MG
EUR 491
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1 20ST
EUR 625
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023 1MG
EUR 281
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025 100uG
EUR 139
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026 500uG
EUR 494
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027 300
EUR 345
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028 100U
EUR 428
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030 300U
EUR 345
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032 300ST
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin

00033 300EU
EUR 345
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M

00034 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M

00034-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP

00036 100ug
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP

00037 100ug
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555

00040 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647

00041 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647

00041-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A

00042 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF488A

00042-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF543

00043 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF543

00043-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF568

00044 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF568

00044-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF594

00045 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF594

00045-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF633

00046 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF633

00046-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660R

00047 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660R

00047-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680R

00048 300U
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680R

00048-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF350

00049 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF350

00049-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF640R

00050 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF640R

00050-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF532

00051 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF532

00051-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF660C

00052 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF660C

00052-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF680

00053 300U
EUR 466
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF680

00053-T 50U
EUR 147
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF430

00054 300U
EUR 449
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF430

00054-T 50U
EUR 139
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF440

00055 300U
EUR 449
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF440

00055-T 50U
EUR 139
Description: Minimum order quantity: 1 unit of 50U

Cholera Toxin Subunit B, CF488A conjugate

00070 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF568 conjugate

00071 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF594 conjugate

00072 100ug
EUR 261
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF640R conjugate

00073 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF532 conjugate

00074 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF543 conjugate

00075 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF620R conjugate

00076 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF633 conjugate

00077 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF660R conjugate

00078 100ug
EUR 264
Description: Minimum order quantity: 1 unit of 100ug

Cholera Toxin Subunit B, CF680R conjugate

00079 100ug
EUR 282
Description: Minimum order quantity: 1 unit of 100ug

Human Transferrin, CF488A conjugate

00081 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF543 conjugate

00082 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF568 conjugate

00083 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF594 conjugate

00084 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

Human Transferrin, CF640R conjugate

00085 1mg
EUR 149
Description: Minimum order quantity: 1 unit of 1mg

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